or placebo o relative to sham instrumented control hearts at top left panel, top right and min bottom left panel mean lvdp of hearts from to min of reperfusion are shown in the bottom right panel tetrazolium chloride stained heart slices showed that hearts treated with csil at , and how to treat low testosterone min had similar injury as that of the flaxseed and warfarin interaction sham operated hearts � , � and � , and � respectively p � ns the infarct size of hearts how to treat low testosterone treated with csil even at min of global ischemia was � of the ventricles this was significantly smaller than its corresponding control p , whereas hearts treated with control iggl at , and min of global ischemia were � , � and � respectively fig , left panel the corresponding nitroblue tetrazolium chloride stained heart how to treat low testosterone slices are shown in fig , right panel another parameter of myocardial injury that was determined was mitochondrial size although mitochondrial swelling is a hallmark of ischemic injury, irreversible injury cannot be directly extrapolated from just observation of mitochondrial size nevertheless, in view of the myocardial functional and his tochemical evidences, mitochondrial size assessment from transmission electron micrographs add additional support for myocardial preservation in csil treated hearts, relative to iggl or placebo treated hearts figure shows the comparison of fig mean infarct sizes of rat hearts treated with csil how to treat low testosterone or iggl or placebo at and min of global ischemia left panel the corresponding nitroblue tetrazolium chloride stained mid slices of rat hearts treated with csil or iggl at , and min of global ischemia minimal injury was seen in and min csil treated hearts, but injury was evident in the min how to treat low testosterone csil treated heart slice injury is evident in all heart slices treated with iggl right panel, bottom two rows �i i normal cut csil csil how to treat low testosterone csejo igsl tg& iflsi placebo v fig mean mitochondrial size of normal, csil, how to treat low testosterone iggl or placebo treated hearts treatment was as indicated in the text mitochondrial size of normal hearts, csil treatment at and min of global ischemia, how to treat low testosterone as well as iggl treated hearts at , and min of global ischemia and with placebo no difference in mitochondrial size was observed between normal myocardium � mean number of pixels � sem and myocardium treated at and min of global ischemia � , � , � and � respectively p = ns however, how to treat low testosterone mitochondria of hearts treated with iggl at , and min of global ischemia or how to treat low testosterone placebo � , � , � and � respectively were larger than mitochondria of csil how to treat low testosterone treated hearts p these studies showed that myocardial viability preservation is not restricted to embryonic cardiocytes in cultures adult hearts are also amenable to structural and functional preservation, following cell membrane lesion sealing in a timedependent manner during ischemia this method of cell membrane lesion sealing has also been reported to preserve the integrity of vascular endothelium with antiactinimmunoliposomes a question that remains concerning the utility of csil is whether immunoliposomes can retain the protective functions how to treat low testosterone in the presence of plasma proteins in vivo, since experiments have demonstrated that cells in culture and adult hearts perfused with nonprotein oxygenated buffer were how to treat low testosterone prevented from undergoing myocardial necrosis, following cell membrane lesion sealing intervention with cytoskeletal how to treat low testosterone antigen specific immunoliposomes to demonstrate that cell membrane lesion sealing also occur in how to treat low testosterone vivo, rabbits with experimental myocardial infarction were used in this study, rabbits how to treat low testosterone were injected with antimyosin csil, plain liposomes or saline at the time of left circumflex coronary artery occlusion by intracoronary infusion the occlusion was kept for min followed by hrs of reperfusion the hearts were excised, sliced into � slices parallel to the short axis and stained with nitroblue tetrazolium chloride the infarct was approximately to of the infarcts of the control plain liposome how to treat low testosterone or saline treated rabbit hearts subsequently, comparison to iggliposome treated hearts with acute myocardial infarction demonstrated that the csil treatment resulted in significantly smaller infarct size, as was observed in comparison to plain liposome or saline treated hearts thus, cytoskeletalantigen specific immunoliposomes, consisting of antimyosin or antiactinimmunoliposomes, were demonstrated to be able to preserve cell viability and integrity its potential utility in the cardiovascular how to treat low testosterone system would be enhanced once its efficacy following intravenous delivery has been demonstrated however, the study of asahi et al showed that intravenous delivery of how to treat low testosterone the antiactin immunoliposomes enabled preservation of the integrity of the endothelial cells of how to treat low testosterone the cerebral vessels csil as targeted gene or drug delivery due to the how to treat low testosterone proposed mechanism of cell membrane lesion sealing, we also proposed that if how to treat low testosterone drugs or gene constructs were to be included in the immunoliposomes such as csils, then these drugs or gene constructs should be delivered directly into the how to treat low testosterone cytoplasm fig this route should bypass the endocytic route of drugs or how to treat low testosterone gene construct delivery, thereby reducing destruction of the delivered cargo by the lysosomal enzymes, after formation of endolysosomes using silver grains as model fig transmission electron micrographs of embryonic cardiocyte treated with silver grains impregnated csil left and plain liposome impregnated with silver grains right � = jim drugs, we demonstrated that these drugs can be delivered directly into the cytoplasm of hypoxic cardiocytes treated with silver grains loaded csils figure left shows a transmission electron micrograph how to treat low testosterone of a cardiocyte treated with silver grains impregnated csils silver grains in groups of concentration at about nm were observed however, in cells treated with how to treat low testosterone silver grains impregnated plain liposomes, very few cells were viable of one such cell detected by transmission electron microscopy, the silver grains were observed in the extracellular space [fig right] fig diagrammatic representation of delivery of intraliposomally entrapped genetic construct or drugs directly into the cytoplasm of target cell nmuin ftivoih how to treat low testosterone wiiit mi ��� when the silver grains were replaced with genetic constructs, pgl and psv gal vectors, hypoxic cardiocytes treated with csil impregnated with either vectors showed luciferase activity or bacterial jgalactosidase activity the successful transfection of the hypoxic cardiocytes with pgl, a vector for firefly luciferase enzyme � � fig how to treat low testosterone relative light units of luciferase activity of cardiocytes treated with various preparations and controls in csils is shown in fig as relative light units rlus rlus were determined by the use of a luminometer as can be seen, only hypoxic cardiocytes treated with pglcsils showed increased rlus significantly above normal cells how to treat low testosterone with treatment with no vectors similarly, normoxic cardiocytes treated with pglcsil, hypoxic cardiocytes and normoxic cardiocytes treated with plain liposomes, or with only vectors, showed no significant gene transcription and expression when hypoxic cardiocytes were treated with csil how to treat low testosterone with entrapped psvgal vectors, almost all cells in the field of view under light microscopy exhibited bacterial �galactosidase enzyme activity, following reaction with xgal bromo how to treat low testosterone chloroindolylbetadgalactopyranoside, nm mgcl,mm iqfetcn h, mm kfecn in phosphate buffered saline ph how to treat low testosterone [fig a] when this mode of gene expression was compared with transfection of how to treat low testosterone psvjgal vector with cationic liposomes, cationic liposome transfection according to the manufacturers protocol resulted in transfection of only a few cells per field of view how to treat low testosterone [fig b], in this micrograph, two cells with intense ��galactosidase activity were observed quantitation of the number of cells in the field of view that was how to treat low testosterone successfully trans fected with psvjgal vector in csil, cationic liposome, iggliposomes, plain how to treat low testosterone liposome and vector alone are shown in fig c only csil and cationic transfection showed gene expression csiltransfection or csilfection was more than times more efficient in transfecting cells than cationic liposomes although the intensity of gene expression was low with csilfection, using the initial vector concentration of xg of vectors in mg lipids in ml, when the vector concentration was increased to � zg, also in mg lipids in ml, the intensity of gene transfection was how to treat low testosterone increased [fig d], this study showed that approximately x xg ��� � m t� !